Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning
Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biolo...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell
2017
|
| Online Access: | http://hdl.handle.net/1721.1/109435 https://orcid.org/0000-0003-0921-3144 |
| _version_ | 1826201942994452480 |
|---|---|
| author | Ozkumur, Ayca Yalcin Love, John C Goods, Brittany A. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ozkumur, Ayca Yalcin Love, John C Goods, Brittany A. |
| author_sort | Ozkumur, Ayca Yalcin |
| collection | MIT |
| description | Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV. |
| first_indexed | 2024-09-23T11:59:44Z |
| format | Article |
| id | mit-1721.1/109435 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:59:44Z |
| publishDate | 2017 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1094352022-10-01T07:30:19Z Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning Ozkumur, Ayca Yalcin Love, John C Goods, Brittany A. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Love, John C Goods, Brittany A. Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV. National Cancer Institute (U.S.) (P30-CA14051) 2017-05-30T19:29:25Z 2017-05-30T19:29:25Z 2015-09 2015-04 Article http://purl.org/eprint/type/JournalArticle 1613-6810 1613-6829 http://hdl.handle.net/1721.1/109435 Ozkumur, Ayca Yalcin; Goods, Brittany A. and Love, J. Christopher. “Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.” Small 11, no. 36 (June 2015): 4643–4650 © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim https://orcid.org/0000-0003-0921-3144 en_US http://dx.doi.org/10.1002/smll.201500674 Small Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Ozkumur, Ayca Yalcin Love, John C Goods, Brittany A. Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning |
| title | Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning |
| title_full | Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning |
| title_fullStr | Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning |
| title_full_unstemmed | Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning |
| title_short | Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning |
| title_sort | development of a high throughput functional screen using nanowell assisted cell patterning |
| url | http://hdl.handle.net/1721.1/109435 https://orcid.org/0000-0003-0921-3144 |
| work_keys_str_mv | AT ozkumuraycayalcin developmentofahighthroughputfunctionalscreenusingnanowellassistedcellpatterning AT lovejohnc developmentofahighthroughputfunctionalscreenusingnanowellassistedcellpatterning AT goodsbrittanya developmentofahighthroughputfunctionalscreenusingnanowellassistedcellpatterning |