Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library

Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library

The Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus is an attractive binding scaffold because of its small size (7 kDa), high thermal stability (Tm of 98 °C), and absence of cysteines and glycosylation sites. However, as a DNA-binding protein, Sso7d is highly positively cha...

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Bibliographic Details
Main Authors: Lobner, Elisabeth, Traxlmayr, Michael, Kiefer, Jonathan, Srinivas, Raja Ram, Tisdale, Alison W., Mehta, Naveen, Yang, Nicole Jie Yeon, Tidor, Bruce, Wittrup, Karl Dane
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: American Society for Biochemistry and Molecular Biology (ASBMB) 2017
Online Access:http://hdl.handle.net/1721.1/109823
https://orcid.org/0000-0002-2108-582X
https://orcid.org/0000-0002-5476-903X
https://orcid.org/0000-0002-3161-2785
https://orcid.org/0000-0003-3480-6750
https://orcid.org/0000-0002-0882-7761
https://orcid.org/0000-0002-3320-3969
https://orcid.org/0000-0003-2398-5896

Internet

http://hdl.handle.net/1721.1/109823
https://orcid.org/0000-0002-2108-582X
https://orcid.org/0000-0002-5476-903X
https://orcid.org/0000-0002-3161-2785
https://orcid.org/0000-0003-3480-6750
https://orcid.org/0000-0002-0882-7761
https://orcid.org/0000-0002-3320-3969
https://orcid.org/0000-0003-2398-5896

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