Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase

Asparagine-linked glycosylation (NLG) plays a significant role in a diverse range of cellular processes, including protein signaling and trafficking, the immunologic response, and immune system evasion by pathogens. A major impediment to NLG-related research is an incomplete understanding of the cen...

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Bibliographic Details
Main Authors: Jaffee, Marcie Beth, Imperiali, Barbara
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:en_US
Published: Elsevier 2017
Online Access:http://hdl.handle.net/1721.1/110397
https://orcid.org/0000-0002-5749-7869