Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase
Asparagine-linked glycosylation (NLG) plays a significant role in a diverse range of cellular processes, including protein signaling and trafficking, the immunologic response, and immune system evasion by pathogens. A major impediment to NLG-related research is an incomplete understanding of the cen...
Main Authors: | Jaffee, Marcie Beth, Imperiali, Barbara |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | en_US |
Published: |
Elsevier
2017
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Online Access: | http://hdl.handle.net/1721.1/110397 https://orcid.org/0000-0002-5749-7869 |
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