mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control
Most eukaryotic mRNAs are recruited to the ribosome by recognition of a 5ʹ m7GpppN cap. 30 years of genetic and biochemical evidence point to a role for interaction between the 5ʹ cap-interacting factors and the 3ʹ poly(A)-binding protein in bringing the ends of the mRNA into close proximity and pro...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Springer-Verlag
2017
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| Online Access: | http://hdl.handle.net/1721.1/110612 https://orcid.org/0000-0001-8281-6916 https://orcid.org/0000-0003-2807-9657 |
| _version_ | 1826189526565912576 |
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| author | Thompson, Mary Katherine Gilbert, Wendy |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Thompson, Mary Katherine Gilbert, Wendy |
| author_sort | Thompson, Mary Katherine |
| collection | MIT |
| description | Most eukaryotic mRNAs are recruited to the ribosome by recognition of a 5ʹ m7GpppN cap. 30 years of genetic and biochemical evidence point to a role for interaction between the 5ʹ cap-interacting factors and the 3ʹ poly(A)-binding protein in bringing the ends of the mRNA into close proximity and promoting both translation and stability of the mRNA, in a form known as the “closed loop”. However, the results of recent RNA–protein interaction studies suggest that not all mRNAs have equal access to the closed loop factors. Furthermore, association with closed loop factors appears to be highly biased towards mRNAs with short open reading frames, echoing the trend for higher translation of short mRNAs that has been observed in many eukaryotes. We recently reported that the ribosomal signaling scaffold protein RACK1 promotes the efficient translation of short mRNAs that strongly associate with the closed loop factors. Here, we discuss the implications of these observations with respect to translational control and suggest avenues through which the universality of the closed loop in eukaryotic translation could be revisited. |
| first_indexed | 2024-09-23T08:16:14Z |
| format | Article |
| id | mit-1721.1/110612 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:16:14Z |
| publishDate | 2017 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | mit-1721.1/1106122022-09-23T12:01:48Z mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control Thompson, Mary Katherine Gilbert, Wendy Massachusetts Institute of Technology. Department of Biology Thompson, Mary Katherine Gilbert, Wendy Most eukaryotic mRNAs are recruited to the ribosome by recognition of a 5ʹ m7GpppN cap. 30 years of genetic and biochemical evidence point to a role for interaction between the 5ʹ cap-interacting factors and the 3ʹ poly(A)-binding protein in bringing the ends of the mRNA into close proximity and promoting both translation and stability of the mRNA, in a form known as the “closed loop”. However, the results of recent RNA–protein interaction studies suggest that not all mRNAs have equal access to the closed loop factors. Furthermore, association with closed loop factors appears to be highly biased towards mRNAs with short open reading frames, echoing the trend for higher translation of short mRNAs that has been observed in many eukaryotes. We recently reported that the ribosomal signaling scaffold protein RACK1 promotes the efficient translation of short mRNAs that strongly associate with the closed loop factors. Here, we discuss the implications of these observations with respect to translational control and suggest avenues through which the universality of the closed loop in eukaryotic translation could be revisited. National Institutes of Health (U.S.) (GM094303) National Institutes of Health (U.S.) (T32GM007287) 2017-07-11T12:40:28Z 2017-10-01T05:00:06Z 2016-12 2016-12 2017-07-11T04:03:10Z Article http://purl.org/eprint/type/JournalArticle 0172-8083 1432-0983 http://hdl.handle.net/1721.1/110612 Thompson, Mary K. and Gilbert, Wendy V. “mRNA Length-Sensing in Eukaryotic Translation: Reconsidering the ‘closed Loop’ and Its Implications for Translational Control.” Current Genetics 63, no. 4 (December 2016): 613–620 © 2016 Springer-Verlag Berlin Heidelberg https://orcid.org/0000-0001-8281-6916 https://orcid.org/0000-0003-2807-9657 en http://dx.doi.org/10.1007/s00294-016-0674-3 Current Genetics Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ Springer-Verlag Berlin Heidelberg application/pdf Springer-Verlag Springer Berlin Heidelberg |
| spellingShingle | Thompson, Mary Katherine Gilbert, Wendy mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control |
| title | mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control |
| title_full | mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control |
| title_fullStr | mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control |
| title_full_unstemmed | mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control |
| title_short | mRNA length-sensing in eukaryotic translation: reconsidering the “closed loop” and its implications for translational control |
| title_sort | mrna length sensing in eukaryotic translation reconsidering the closed loop and its implications for translational control |
| url | http://hdl.handle.net/1721.1/110612 https://orcid.org/0000-0001-8281-6916 https://orcid.org/0000-0003-2807-9657 |
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