Improved vectors and genome-wide libraries for CRISPR screening

To the Editor: Genome-wide, targeted loss-of-function pooled screens using the clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi)1, 2, 3, 4. Previously, we used a g...

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Bibliographic Details
Main Authors: Sanjana, Neville E, Shalem, Ophir, Zhang, Feng
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: Springer Nature 2017
Online Access:http://hdl.handle.net/1721.1/110852
https://orcid.org/0000-0003-2782-2509