Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB
Age is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevate...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Springer Nature
2017
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| Online Access: | http://hdl.handle.net/1721.1/110863 |
| _version_ | 1811078992192602112 |
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| author | Haustead, Daniel J. Stevenson, Andrew Saxena, Vishal Marriage, Fiona Firth, Martin Silla, Robyn Martin, Lisa Adcroft, Katharine F. Rea, Suzanne Day, Philip J. Melton, Phillip Wood, Fiona M. Fear, Mark W. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Haustead, Daniel J. Stevenson, Andrew Saxena, Vishal Marriage, Fiona Firth, Martin Silla, Robyn Martin, Lisa Adcroft, Katharine F. Rea, Suzanne Day, Philip J. Melton, Phillip Wood, Fiona M. Fear, Mark W. |
| author_sort | Haustead, Daniel J. |
| collection | MIT |
| description | Age is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevated, transcriptome-wide expression changes occurring predominantly in middle age. Both pre and post this period, the transcriptome appears to undergo much smaller, linear changes with increasing age. Functional analysis of the transient changes in middle age suggest a period of heightened metabolic activity and cellular damage associated with NF-kappa-B and TNF signaling pathways. Through meta-analysis we also show the presence of global, tissue independent linear transcriptome changes with age which appear to be regulated by NF-kappa-B. These results suggest that aging in human skin is associated with a critical mid-life period with widespread transcriptome changes, both preceded and proceeded by a relatively steady rate of linear change in the transcriptome. The data provides insight into molecular changes associated with normal aging and will help to better understand the increasingly important pathological changes associated with aging. |
| first_indexed | 2024-09-23T11:08:27Z |
| format | Article |
| id | mit-1721.1/110863 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:08:27Z |
| publishDate | 2017 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1108632022-10-01T01:34:04Z Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB Haustead, Daniel J. Stevenson, Andrew Saxena, Vishal Marriage, Fiona Firth, Martin Silla, Robyn Martin, Lisa Adcroft, Katharine F. Rea, Suzanne Day, Philip J. Melton, Phillip Wood, Fiona M. Fear, Mark W. Massachusetts Institute of Technology. Department of Mechanical Engineering Saxena, Vishal Age is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevated, transcriptome-wide expression changes occurring predominantly in middle age. Both pre and post this period, the transcriptome appears to undergo much smaller, linear changes with increasing age. Functional analysis of the transient changes in middle age suggest a period of heightened metabolic activity and cellular damage associated with NF-kappa-B and TNF signaling pathways. Through meta-analysis we also show the presence of global, tissue independent linear transcriptome changes with age which appear to be regulated by NF-kappa-B. These results suggest that aging in human skin is associated with a critical mid-life period with widespread transcriptome changes, both preceded and proceeded by a relatively steady rate of linear change in the transcriptome. The data provides insight into molecular changes associated with normal aging and will help to better understand the increasingly important pathological changes associated with aging. 2017-07-26T19:46:52Z 2017-07-26T19:46:52Z 2016-05 2015-10 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/110863 Haustead, Daniel J., Andrew Stevenson, Vishal Saxena, Fiona Marriage, Martin Firth, Robyn Silla, Lisa Martin, et al. “Transcriptome Analysis of Human Ageing in Male Skin Shows Mid-Life Period of Variability and Central Role of NF-κB.” Scientific Reports 6 (May 27, 2016): 26846. en_US http://dx.doi.org/10.1038/srep26846 Scientific Reports Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Springer Nature Scientific Reports |
| spellingShingle | Haustead, Daniel J. Stevenson, Andrew Saxena, Vishal Marriage, Fiona Firth, Martin Silla, Robyn Martin, Lisa Adcroft, Katharine F. Rea, Suzanne Day, Philip J. Melton, Phillip Wood, Fiona M. Fear, Mark W. Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB |
| title | Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB |
| title_full | Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB |
| title_fullStr | Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB |
| title_full_unstemmed | Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB |
| title_short | Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB |
| title_sort | transcriptome analysis of human ageing in male skin shows mid life period of variability and central role of nf κb |
| url | http://hdl.handle.net/1721.1/110863 |
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