High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption
Nuclear transfection of DNA into mammalian cells is challenging yet critical for many biological and medical studies. Here, by combining cell squeezing and electric-field-driven transport in a device that integrates microfluidic channels with constrictions and microelectrodes, we demonstrate nuclear...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/1721.1/111022 https://orcid.org/0000-0003-4252-9335 https://orcid.org/0000-0003-4112-6622 https://orcid.org/0000-0002-9016-5962 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-7192-580X |