Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic repeats)–associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockou...

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Bibliographic Details
Main Authors: Hartenian, E., Shi, X., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., Shalem, Ophir, Sanjana, Neville E, Scott, David Arthur, Zhang, Feng
Other Authors: McGovern Institute for Brain Research at MIT
Format: Article
Language:en_US
Published: American Association for the Advancement of Science (AAAS) 2017
Online Access:http://hdl.handle.net/1721.1/111576
https://orcid.org/0000-0002-2639-9879
https://orcid.org/0000-0003-2782-2509

Internet

http://hdl.handle.net/1721.1/111576
https://orcid.org/0000-0002-2639-9879
https://orcid.org/0000-0003-2782-2509

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