Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

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The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 Å resolution. The structure revealed a bilobed architecture composed of...

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Bibliographic Details
Main Authors: Nishimasu, Hiroshi, Dohmae, Naoshi, Ishitani, Ryuichiro, Nureki, Osamu, Ran, Fei, Hsu, Patrick, Konermann, Silvana M, Shehata, Soraya I., Zhang, Feng
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Published: Elsevier 2017
Online Access:http://hdl.handle.net/1721.1/112727
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0003-2782-2509

Internet

http://hdl.handle.net/1721.1/112727
https://orcid.org/0000-0001-7915-1685
https://orcid.org/0000-0003-2782-2509

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