Engineered Cpf1 variants with altered PAM specificities

Engineered Cpf1 variants with altered PAM specificities

The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells. However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM)...

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Bibliographic Details
Main Authors: Schneider, Martin W, Yamano, Takashi, Nishimasu, Hiroshi, Nureki, Osamu, Crosetto, Nicola, Gao, Linyi, Cox, David Benjamin Turitz, Yan, Winston Xia, Manteiga, John Colonnese, Zhang, Feng
Other Authors: Institute for Medical Engineering and Science
Format: Article
Published: Nature Publishing Group 2018
Online Access:http://hdl.handle.net/1721.1/113321
https://orcid.org/0000-0002-3579-0327
https://orcid.org/0000-0001-7626-4254
https://orcid.org/0000-0002-3067-479X
https://orcid.org/0000-0002-0385-6176
https://orcid.org/0000-0003-2782-2509

Internet

http://hdl.handle.net/1721.1/113321
https://orcid.org/0000-0002-3579-0327
https://orcid.org/0000-0001-7626-4254
https://orcid.org/0000-0002-3067-479X
https://orcid.org/0000-0002-0385-6176
https://orcid.org/0000-0003-2782-2509

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