Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction

Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly-adenylated transcripts or target...

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Bibliographic Details
Main Authors: Herbert, Zachary T, Kershner, Jamie P, Thimmapuram, Jyothi, Choudhari, Sulbha, Alekseyev, Yuriy O, Fan, Jun, Podnar, Jessica W, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra S, Morris, Krystalynne, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen D, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Butty, Vincent L G, Levine, Stuart S.
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Language:English
Published: BioMed Central Ltd 2018
Online Access:http://hdl.handle.net/1721.1/114726