Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction
Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly-adenylated transcripts or target...
Main Authors: | Herbert, Zachary T, Kershner, Jamie P, Thimmapuram, Jyothi, Choudhari, Sulbha, Alekseyev, Yuriy O, Fan, Jun, Podnar, Jessica W, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra S, Morris, Krystalynne, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen D, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Butty, Vincent L G, Levine, Stuart S. |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | English |
Published: |
BioMed Central Ltd
2018
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Online Access: | http://hdl.handle.net/1721.1/114726 |
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