Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and
Vaccines have had broad medical impact, but existing vaccine technologies and production methods are limited in their ability to respond rapidly to evolving and emerging pathogens, or sudden outbreaks. Here, we develop a rapid-response, fully synthetic, singledose, adjuvant-free dendrimer nanopartic...
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| Format: | Article |
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National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/114728 https://orcid.org/0000-0003-3811-2369 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-5629-4798 |
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| author | Chahal, Jasdave S. Cooper, Christopher L. McPartlan, Justine S. Tilley, Lucas D. Sidik, Saima M. Lourido, Sebastian Bavari, Sina Ploegh, Hidde L. Khan, Omar Fizal Tsosie, Jonathan Langer, Robert S Anderson, Daniel Griffith |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Chahal, Jasdave S. Cooper, Christopher L. McPartlan, Justine S. Tilley, Lucas D. Sidik, Saima M. Lourido, Sebastian Bavari, Sina Ploegh, Hidde L. Khan, Omar Fizal Tsosie, Jonathan Langer, Robert S Anderson, Daniel Griffith |
| author_sort | Chahal, Jasdave S. |
| collection | MIT |
| description | Vaccines have had broad medical impact, but existing vaccine technologies and production methods are limited in their ability to respond rapidly to evolving and emerging pathogens, or sudden outbreaks. Here, we develop a rapid-response, fully synthetic, singledose, adjuvant-free dendrimer nanoparticle vaccine platform wherein antigens are encoded by encapsulated mRNA replicons. To our knowledge, this system is the first capable of generating protective immunity against a broad spectrum of lethal pathogen challenges, including H1N1 influenza, Toxoplasma gondii, and Ebola virus. The vaccine can be formed with multiple antigenexpressing replicons, and is capable of eliciting both CD8⁺ T-cell and antibody responses. The ability to generate viable, contaminant-free vaccines within days, to single or multiple antigens, may have broad utility for a range of diseases. |
| first_indexed | 2024-09-23T09:41:28Z |
| format | Article |
| id | mit-1721.1/114728 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T09:41:28Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1147282022-09-30T16:13:11Z Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Chahal, Jasdave S. Cooper, Christopher L. McPartlan, Justine S. Tilley, Lucas D. Sidik, Saima M. Lourido, Sebastian Bavari, Sina Ploegh, Hidde L. Khan, Omar Fizal Tsosie, Jonathan Langer, Robert S Anderson, Daniel Griffith Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Khan, Omar Fizal Tsosie, Jonathan Langer, Robert S Anderson, Daniel Griffith Vaccines have had broad medical impact, but existing vaccine technologies and production methods are limited in their ability to respond rapidly to evolving and emerging pathogens, or sudden outbreaks. Here, we develop a rapid-response, fully synthetic, singledose, adjuvant-free dendrimer nanoparticle vaccine platform wherein antigens are encoded by encapsulated mRNA replicons. To our knowledge, this system is the first capable of generating protective immunity against a broad spectrum of lethal pathogen challenges, including H1N1 influenza, Toxoplasma gondii, and Ebola virus. The vaccine can be formed with multiple antigenexpressing replicons, and is capable of eliciting both CD8⁺ T-cell and antibody responses. The ability to generate viable, contaminant-free vaccines within days, to single or multiple antigens, may have broad utility for a range of diseases. 2018-04-13T19:38:07Z 2018-04-13T19:38:07Z 2016-07 2016-01 2018-04-13T19:15:52Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/114728 Chahal, Jasdave S. et al. “Dendrimer-RNA Nanoparticles Generate Protective Immunity Against Lethal Ebola, H1N1 Influenza, andToxoplasma Gondiichallenges with a Single Dose.” Proceedings of the National Academy of Sciences 113, 29 (July 2016): E4133–E4142 © 2016 National Academy of Sciences https://orcid.org/0000-0003-3811-2369 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-5629-4798 http://dx.doi.org/10.1073/PNAS.1600299113 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Sciences |
| spellingShingle | Chahal, Jasdave S. Cooper, Christopher L. McPartlan, Justine S. Tilley, Lucas D. Sidik, Saima M. Lourido, Sebastian Bavari, Sina Ploegh, Hidde L. Khan, Omar Fizal Tsosie, Jonathan Langer, Robert S Anderson, Daniel Griffith Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and |
| title | Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and |
| title_full | Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and |
| title_fullStr | Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and |
| title_full_unstemmed | Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and |
| title_short | Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and |
| title_sort | dendrimer rna nanoparticles generate protective immunity against lethal ebola h1n1 influenza and |
| url | http://hdl.handle.net/1721.1/114728 https://orcid.org/0000-0003-3811-2369 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0001-5629-4798 |
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