SMC complexes differentially compact mitotic chromosomes according to genomic context
Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical r...
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| Format: | Article |
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Nature Publishing Group
2018
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| Online Access: | http://hdl.handle.net/1721.1/114831 https://orcid.org/0000-0002-2210-8616 https://orcid.org/0000-0001-5905-6517 https://orcid.org/0000-0002-0785-5410 |
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| author | Schalbetter, Stephanie Andrea Belton, Jon-Matthew Miles, Catrina Yu, Miao Dekker, Job Baxter, Jonathan Goloborodko, Anton Fudenberg, Geoffrey Mirny, Leonid A |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science Schalbetter, Stephanie Andrea Belton, Jon-Matthew Miles, Catrina Yu, Miao Dekker, Job Baxter, Jonathan Goloborodko, Anton Fudenberg, Geoffrey Mirny, Leonid A |
| author_sort | Schalbetter, Stephanie Andrea |
| collection | MIT |
| description | Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes. |
| first_indexed | 2024-09-23T10:50:40Z |
| format | Article |
| id | mit-1721.1/114831 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T10:50:40Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1148312022-09-30T23:25:51Z SMC complexes differentially compact mitotic chromosomes according to genomic context SMC complexes differentially compact mitotic chromosomes according to genomic context Schalbetter, Stephanie Andrea Belton, Jon-Matthew Miles, Catrina Yu, Miao Dekker, Job Baxter, Jonathan Goloborodko, Anton Fudenberg, Geoffrey Mirny, Leonid A Institute for Medical Engineering and Science Massachusetts Institute of Technology. Department of Physics Goloborodko, Anton Fudenberg, Geoffrey Mirny, Leonid A Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes. National Institutes of Health (U.S.) (Grant R01HG003143) 2018-04-20T20:04:56Z 2018-04-20T20:04:56Z 2017-08 2017-03 2018-04-19T15:05:47Z Article http://purl.org/eprint/type/JournalArticle 1465-7392 1476-4679 http://hdl.handle.net/1721.1/114831 Schalbetter, Stephanie Andrea et al “SMC Complexes Differentially Compact Mitotic Chromosomes According to Genomic Context.” Nature Cell Biology 19, 9 (August 2017): 1071–1080 © 2017 Macmillan Publishers Limited, part of Springer Nature https://orcid.org/0000-0002-2210-8616 https://orcid.org/0000-0001-5905-6517 https://orcid.org/0000-0002-0785-5410 http://dx.doi.org/10.1038/NCB3594 Nature Cell Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Schalbetter, Stephanie Andrea Belton, Jon-Matthew Miles, Catrina Yu, Miao Dekker, Job Baxter, Jonathan Goloborodko, Anton Fudenberg, Geoffrey Mirny, Leonid A SMC complexes differentially compact mitotic chromosomes according to genomic context |
| title | SMC complexes differentially compact mitotic chromosomes according to genomic context |
| title_full | SMC complexes differentially compact mitotic chromosomes according to genomic context |
| title_fullStr | SMC complexes differentially compact mitotic chromosomes according to genomic context |
| title_full_unstemmed | SMC complexes differentially compact mitotic chromosomes according to genomic context |
| title_short | SMC complexes differentially compact mitotic chromosomes according to genomic context |
| title_sort | smc complexes differentially compact mitotic chromosomes according to genomic context |
| url | http://hdl.handle.net/1721.1/114831 https://orcid.org/0000-0002-2210-8616 https://orcid.org/0000-0001-5905-6517 https://orcid.org/0000-0002-0785-5410 |
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