Effect of directional pulling on mechanical protein degradation by ATP-dependent proteolytic machines
AAA+ proteases and remodeling machines couple hydrolysis of ATP to mechanical unfolding and translocation of proteins following recognition of sequence tags called degrons. Here, we use single-molecule optical trapping to determine the mechanochemistry of two AAA+ proteases, Escherichia coli ClpXP a...
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
National Academy of Sciences (U.S.)
2018
|
Online Access: | http://hdl.handle.net/1721.1/114914 https://orcid.org/0000-0001-9751-9535 https://orcid.org/0000-0002-2246-2674 https://orcid.org/0000-0002-1719-5399 |