Scarless Cas9 Assisted Recombineering (no‐SCAR) in Escherichia coli, an Easy‐to‐Use System for Genome Editing
The discovery and development of genome editing systems that leverage the site‐specific DNA endonuclease system CRISPR/Cas9 has fundamentally changed the ease and speed of genome editing in many organisms. In eukaryotes, the CRISPR/Cas9 system utilizes a “guide” RNA to enable the Cas9 nuclease to ma...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2018
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| Online Access: | http://hdl.handle.net/1721.1/115128 https://orcid.org/0000-0002-4908-3914 https://orcid.org/0000-0003-0437-3157 |