A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
We describe a simple, robust and high throughput single molecule flow-stretching assay for studying 1D diffusion of molecules along DNA. In this assay, glass coverslips are functionalized in a one-step reaction with silane-PEG-biotin. Flow cells are constructed by sandwiching an adhesive tape with p...
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| Format: | Article |
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MyJove Corporation
2018
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| Online Access: | http://hdl.handle.net/1721.1/115428 https://orcid.org/0000-0001-7014-3830 |