Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Genetic screens help infer gene function in mammalian cells, but it has remained difficult to assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop Perturb-seq, combining single-cell RNA sequencing (RNA-seq) and clustered regularly interspaced short palindromic repeats...

Full description

Bibliographic Details
Main Authors: Dixit, Atray, Parnas, Oren, Li, Biyu, Chen, Jenny, Fulco, Charles P., Jerby-Arnon, Livnat, Marjanovic, Nemanja D., Dionne, Danielle, Burks, Tyler, Raychowdhury, Raktima, Adamson, Britt, Norman, Thomas M., Weissman, Jonathan S., Friedman, Nir, Lander, Eric Steven, Regev, Aviv
Other Authors: Massachusetts Institute of Technology. Department of Biology
Format: Article
Published: Elsevier BV 2018
Online Access:http://hdl.handle.net/1721.1/116701
https://orcid.org/0000-0001-8567-2049

Internet

http://hdl.handle.net/1721.1/116701
https://orcid.org/0000-0001-8567-2049

Similar Items