A Phase Separation Model for Transcriptional Control
Phase-separated multi-molecular assemblies provide a general regulatory mechanism to compartmentalize biochemical reactions within cells. We propose that a phase separation model explains established and recently described features of transcriptional control. These features include the formation of...
| Main Authors: | , , , , |
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| Format: | Article |
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Elsevier
2018
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| Online Access: | http://hdl.handle.net/1721.1/116858 https://orcid.org/0000-0002-4167-9385 https://orcid.org/0000-0001-8855-8647 https://orcid.org/0000-0003-1268-9602 https://orcid.org/0000-0003-1465-1691 |
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| author | Hnisz, Denes Young, Richard A. Chakraborty, Arup K. Sharp, Phillip A. Shrinivas, Krishna,Ph. D.Massachusetts Institute of Technology. |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Hnisz, Denes Young, Richard A. Chakraborty, Arup K. Sharp, Phillip A. Shrinivas, Krishna,Ph. D.Massachusetts Institute of Technology. |
| author_sort | Hnisz, Denes |
| collection | MIT |
| description | Phase-separated multi-molecular assemblies provide a general regulatory mechanism to compartmentalize biochemical reactions within cells. We propose that a phase separation model explains established and recently described features of transcriptional control. These features include the formation of super-enhancers, the sensitivity of super-enhancers to perturbation, the transcriptional bursting patterns of enhancers, and the ability of an enhancer to produce simultaneous activation at multiple genes. This model provides a conceptual framework to further explore principles of gene control in mammals. Keywords: super-enhancer; enhancer; phase separation; transcription; nuclear body; gene control; bursting; transcriptional burst; co-operativity |
| first_indexed | 2024-09-23T10:44:01Z |
| format | Article |
| id | mit-1721.1/116858 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T10:44:01Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1168582022-09-30T22:37:30Z A Phase Separation Model for Transcriptional Control Hnisz, Denes Young, Richard A. Chakraborty, Arup K. Sharp, Phillip A. Shrinivas, Krishna,Ph. D.Massachusetts Institute of Technology. Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Physics Koch Institute for Integrative Cancer Research at MIT Shrinivas, Krishna Young, Richard A. Chakraborty, Arup K. Sharp, Phillip A. Phase-separated multi-molecular assemblies provide a general regulatory mechanism to compartmentalize biochemical reactions within cells. We propose that a phase separation model explains established and recently described features of transcriptional control. These features include the formation of super-enhancers, the sensitivity of super-enhancers to perturbation, the transcriptional bursting patterns of enhancers, and the ability of an enhancer to produce simultaneous activation at multiple genes. This model provides a conceptual framework to further explore principles of gene control in mammals. Keywords: super-enhancer; enhancer; phase separation; transcription; nuclear body; gene control; bursting; transcriptional burst; co-operativity National Institutes of Health (U.S.) (Grant HG002668) National Institutes of Health (U.S.) (Grant P01-CA042063) National Cancer Institute (U.S.) (Grant P30-CA14051) 2018-07-09T17:57:51Z 2018-07-09T17:57:51Z 2017-03 2018-07-09T15:36:21Z Article http://purl.org/eprint/type/JournalArticle 0092-8674 1097-4172 http://hdl.handle.net/1721.1/116858 Hnisz, Denes, et al. “A Phase Separation Model for Transcriptional Control.” Cell 169, 1 (March 2017): 13–23 © 2017 Elsevier Inc https://orcid.org/0000-0002-4167-9385 https://orcid.org/0000-0001-8855-8647 https://orcid.org/0000-0003-1268-9602 https://orcid.org/0000-0003-1465-1691 http://dx.doi.org/10.1016/J.CELL.2017.02.007 Cell Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Hnisz, Denes Young, Richard A. Chakraborty, Arup K. Sharp, Phillip A. Shrinivas, Krishna,Ph. D.Massachusetts Institute of Technology. A Phase Separation Model for Transcriptional Control |
| title | A Phase Separation Model for Transcriptional Control |
| title_full | A Phase Separation Model for Transcriptional Control |
| title_fullStr | A Phase Separation Model for Transcriptional Control |
| title_full_unstemmed | A Phase Separation Model for Transcriptional Control |
| title_short | A Phase Separation Model for Transcriptional Control |
| title_sort | phase separation model for transcriptional control |
| url | http://hdl.handle.net/1721.1/116858 https://orcid.org/0000-0002-4167-9385 https://orcid.org/0000-0001-8855-8647 https://orcid.org/0000-0003-1268-9602 https://orcid.org/0000-0003-1465-1691 |
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