Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis
Skeletal metastases, the leading cause of death in advanced breast cancer patients, depend on tumor cell interactions with the mineralized bone extracellular matrix. Bone mineral is largely composed of hydroxyapatite (HA) nanocrystals with physicochemical properties that vary significantly by anatom...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
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National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/117406 https://orcid.org/0000-0001-6026-3433 https://orcid.org/0000-0002-1207-4926 |
| _version_ | 1811092196591403008 |
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| author | He, Frank Chiou, Aaron E. Loh, Hyun Chae Lynch, Maureen Seo, Bo Ri Song, Young Hye Lee, Min Joon Hoerth, Rebecca Bortel, Emely L. Willie, Bettina M. Duda, Georg N. Estroff, Lara A. Masic, Admir Wagermaier, Wolfgang Fratzl, Peter Fischbach, Claudia |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering He, Frank Chiou, Aaron E. Loh, Hyun Chae Lynch, Maureen Seo, Bo Ri Song, Young Hye Lee, Min Joon Hoerth, Rebecca Bortel, Emely L. Willie, Bettina M. Duda, Georg N. Estroff, Lara A. Masic, Admir Wagermaier, Wolfgang Fratzl, Peter Fischbach, Claudia |
| author_sort | He, Frank |
| collection | MIT |
| description | Skeletal metastases, the leading cause of death in advanced breast cancer patients, depend on tumor cell interactions with the mineralized bone extracellular matrix. Bone mineral is largely composed of hydroxyapatite (HA) nanocrystals with physicochemical properties that vary significantly by anatomical location, age, and pathology. However, it remains unclear whether bone regions typically targeted by metastatic breast cancer feature distinct HA materials properties. Here we combined high-resolution X-ray scattering analysis with large-area Raman imaging, backscattered electron microscopy, histopathology, and microcomputed tomography to characterize HA in mouse models of advanced breast cancer in relevant skeletal locations. The proximal tibial metaphysis served as a common metastatic site in our studies; we identified that in disease-free bones this skeletal region contained smaller and less-oriented HA nanocrystals relative to ones that constitute the diaphysis. We further observed that osteolytic bone metastasis led to a decrease in HA nanocrystal size and perfection in remnant metaphyseal trabecular bone. Interestingly, in a model of localized breast cancer, metaphyseal HA nanocrystals were also smaller and less perfect than in corresponding bone in disease-free controls. Collectively, these results suggest that skeletal sites prone to tumor cell dissemination contain less-mature HA (i.e., smaller, less-perfect, and less-oriented crystals) and that primary tumors can further increase HA immaturity even before secondary tumor formation, mimicking alterations present during tibial metastasis. Engineered tumor models recapitulating these spatiotemporal dynamics will permit assessing the functional relevance of the detected changes to the progression and treatment of breast cancer bone metastasis. Keywords: breast cancer; bone metastasis; bone mineral nanostructure; X-ray scattering; Raman imaging |
| first_indexed | 2024-09-23T15:14:12Z |
| format | Article |
| id | mit-1721.1/117406 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:14:12Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1174062022-10-02T01:35:22Z Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis He, Frank Chiou, Aaron E. Loh, Hyun Chae Lynch, Maureen Seo, Bo Ri Song, Young Hye Lee, Min Joon Hoerth, Rebecca Bortel, Emely L. Willie, Bettina M. Duda, Georg N. Estroff, Lara A. Masic, Admir Wagermaier, Wolfgang Fratzl, Peter Fischbach, Claudia Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Loh, Hyun Chae Masic, Admir Skeletal metastases, the leading cause of death in advanced breast cancer patients, depend on tumor cell interactions with the mineralized bone extracellular matrix. Bone mineral is largely composed of hydroxyapatite (HA) nanocrystals with physicochemical properties that vary significantly by anatomical location, age, and pathology. However, it remains unclear whether bone regions typically targeted by metastatic breast cancer feature distinct HA materials properties. Here we combined high-resolution X-ray scattering analysis with large-area Raman imaging, backscattered electron microscopy, histopathology, and microcomputed tomography to characterize HA in mouse models of advanced breast cancer in relevant skeletal locations. The proximal tibial metaphysis served as a common metastatic site in our studies; we identified that in disease-free bones this skeletal region contained smaller and less-oriented HA nanocrystals relative to ones that constitute the diaphysis. We further observed that osteolytic bone metastasis led to a decrease in HA nanocrystal size and perfection in remnant metaphyseal trabecular bone. Interestingly, in a model of localized breast cancer, metaphyseal HA nanocrystals were also smaller and less perfect than in corresponding bone in disease-free controls. Collectively, these results suggest that skeletal sites prone to tumor cell dissemination contain less-mature HA (i.e., smaller, less-perfect, and less-oriented crystals) and that primary tumors can further increase HA immaturity even before secondary tumor formation, mimicking alterations present during tibial metastasis. Engineered tumor models recapitulating these spatiotemporal dynamics will permit assessing the functional relevance of the detected changes to the progression and treatment of breast cancer bone metastasis. Keywords: breast cancer; bone metastasis; bone mineral nanostructure; X-ray scattering; Raman imaging National Institutes of Health (U.S.) (Grant R01CA173083) National Cancer Institute (U.S.) (Grant 1U54CA199081-01) National Cancer Institute (U.S.) (Grant 1U54CA210184-01) 2018-08-20T13:39:41Z 2018-08-20T13:39:41Z 2017-08 2017-05 2018-08-20T11:31:49Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/117406 He, Frank et al. “Multiscale Characterization of the Mineral Phase at Skeletal Sites of Breast Cancer Metastasis.” Proceedings of the National Academy of Sciences 114, 40 (September 2017): 10542–10547 © 2017 National Academy of Sciences https://orcid.org/0000-0001-6026-3433 https://orcid.org/0000-0002-1207-4926 http://dx.doi.org/10.1073/PNAS.1708161114 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | He, Frank Chiou, Aaron E. Loh, Hyun Chae Lynch, Maureen Seo, Bo Ri Song, Young Hye Lee, Min Joon Hoerth, Rebecca Bortel, Emely L. Willie, Bettina M. Duda, Georg N. Estroff, Lara A. Masic, Admir Wagermaier, Wolfgang Fratzl, Peter Fischbach, Claudia Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| title | Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| title_full | Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| title_fullStr | Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| title_full_unstemmed | Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| title_short | Multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| title_sort | multiscale characterization of the mineral phase at skeletal sites of breast cancer metastasis |
| url | http://hdl.handle.net/1721.1/117406 https://orcid.org/0000-0001-6026-3433 https://orcid.org/0000-0002-1207-4926 |
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