In vitro synthesis of gene-length single-stranded DNA
Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for enzymatic...
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| Format: | Article |
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Nature Publishing Group
2018
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| Online Access: | http://hdl.handle.net/1721.1/117554 https://orcid.org/0000-0002-2726-3770 https://orcid.org/0000-0001-7122-1917 https://orcid.org/0000-0002-1766-807X https://orcid.org/0000-0002-6199-6855 |