Modification of proteolytic activity matrix analysis (PrAMA) to measure ADAM10 and ADAM17 sheddase activities in cell and tissue lysates

Modification of proteolytic activity matrix analysis (PrAMA) to measure ADAM10 and ADAM17 sheddase activities in cell and tissue lysates

Increases in expression of ADAM10 and ADAM17 genes and proteins have been evaluated, but not validated as cancer biomarkers. Specific enzyme activities better reflect enzyme cellular functions, and might be better biomarkers than enzyme genes or proteins. However, no high throughput assay is availab...

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Bibliographic Details
Main Authors: Yoneyama, Toshie, Gorry, Michael, Gaither-Davis, Autumn, Lin, Yan, Moss, Marcia L., Stabile, Laura P., Herman, James G., Vujanovic, Nikola L., Miller, Miles Aaron, Griffith, Linda G, Lauffenburger, Douglas A
Other Authors: Massachusetts Institute of Technology. Biotechnology Process Engineering Center
Format: Article
Published: Ivyspring International Publisher 2018
Online Access:http://hdl.handle.net/1721.1/117613
https://orcid.org/0000-0002-1801-5548
https://orcid.org/0000-0002-0050-989X

Internet

http://hdl.handle.net/1721.1/117613
https://orcid.org/0000-0002-1801-5548
https://orcid.org/0000-0002-0050-989X

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