Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites
Mitochondria carry out numerous metabolic reactions that are critical to cellular homeostasis. Here we present a protocol for interrogating mitochondrial metabolites and measuring their matrix concentrations. Our workflow uses high-affinity magnetic immunocapture to rapidly purify HA-tagged mitochon...
| Main Authors: | , , |
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| Format: | Article |
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Springer Nature
2018
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| Online Access: | http://hdl.handle.net/1721.1/118755 https://orcid.org/0000-0002-7043-5013 https://orcid.org/0000-0002-1446-7256 |