6 Principles and applications of temporal-focusing wide-field two-photon microscopy
Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width i...
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Format: | Article |
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2019
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Online Access: | http://hdl.handle.net/1721.1/119857 https://orcid.org/0000-0003-4698-6488 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0002-8261-2371 |