6 Principles and applications of temporal-focusing wide-field two-photon microscopy

Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width i...

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Bibliographic Details
Main Authors: So, Peter T. C., Choi, Heejin, Yew, Yan Seng Elijah, Rowlands, Christopher
Other Authors: Massachusetts Institute of Technology. Department of Mechanical Engineering
Format: Article
Published: 2019
Online Access:http://hdl.handle.net/1721.1/119857
https://orcid.org/0000-0003-4698-6488
https://orcid.org/0000-0003-3681-7410
https://orcid.org/0000-0002-8261-2371