6 Principles and applications of temporal-focusing wide-field two-photon microscopy
Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width i...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Published: |
2019
|
| Online Access: | http://hdl.handle.net/1721.1/119857 https://orcid.org/0000-0003-4698-6488 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0002-8261-2371 |
| _version_ | 1826204973761822720 |
|---|---|
| author | So, Peter T. C. Choi, Heejin Yew, Yan Seng Elijah Rowlands, Christopher |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering So, Peter T. C. Choi, Heejin Yew, Yan Seng Elijah Rowlands, Christopher |
| author_sort | So, Peter T. C. |
| collection | MIT |
| description | Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width is achieved by diffracting the light off a grating resulting in spectral component separation and temporal broadening; these spectral components are only recombined at the focal plane to reproduce short, femtosecond pulses. Applications include (i) high speed functional imaging in the brain, (ii) fast FLIM and PLIM, (iii) cell-selective optogenetic excitation, and (iv) temporal focusing photodynamic therapy that may allow selective killing of cancer cells. |
| first_indexed | 2024-09-23T13:05:05Z |
| format | Article |
| id | mit-1721.1/119857 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:05:05Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | mit-1721.1/1198572022-09-28T11:53:40Z 6 Principles and applications of temporal-focusing wide-field two-photon microscopy So, Peter T. C. Choi, Heejin Yew, Yan Seng Elijah Rowlands, Christopher Massachusetts Institute of Technology. Department of Mechanical Engineering So, Peter T. C. Choi, Heejin Yew, Yan Seng Elijah Rowlands, Christopher Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width is achieved by diffracting the light off a grating resulting in spectral component separation and temporal broadening; these spectral components are only recombined at the focal plane to reproduce short, femtosecond pulses. Applications include (i) high speed functional imaging in the brain, (ii) fast FLIM and PLIM, (iii) cell-selective optogenetic excitation, and (iv) temporal focusing photodynamic therapy that may allow selective killing of cancer cells. 2019-01-04T18:38:14Z 2019-01-04T18:38:14Z 2018-01 2019-01-04T14:12:16Z Article http://purl.org/eprint/type/JournalArticle 9783110438987 http://hdl.handle.net/1721.1/119857 So, Peter T. C., Heejin Choi, Elijah Yew, and Christopher Rowlands. “6 Principles and Applications of Temporal-Focusing Wide-Field Two-Photon Microscopy.” Multiphoton Microscopy and Fluorescence Lifetime Imaging, edited by Karsten König, 2018, pp. 103–140. © 2018 Peter T. C. So et al. https://orcid.org/0000-0003-4698-6488 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0002-8261-2371 http://dx.doi.org/10.1515/9783110429985-008 Multiphoton Microscopy and Fluorescence Lifetime Imaging Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf De Gruyter |
| spellingShingle | So, Peter T. C. Choi, Heejin Yew, Yan Seng Elijah Rowlands, Christopher 6 Principles and applications of temporal-focusing wide-field two-photon microscopy |
| title | 6 Principles and applications of temporal-focusing wide-field two-photon microscopy |
| title_full | 6 Principles and applications of temporal-focusing wide-field two-photon microscopy |
| title_fullStr | 6 Principles and applications of temporal-focusing wide-field two-photon microscopy |
| title_full_unstemmed | 6 Principles and applications of temporal-focusing wide-field two-photon microscopy |
| title_short | 6 Principles and applications of temporal-focusing wide-field two-photon microscopy |
| title_sort | 6 principles and applications of temporal focusing wide field two photon microscopy |
| url | http://hdl.handle.net/1721.1/119857 https://orcid.org/0000-0003-4698-6488 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0002-8261-2371 |
| work_keys_str_mv | AT sopetertc 6principlesandapplicationsoftemporalfocusingwidefieldtwophotonmicroscopy AT choiheejin 6principlesandapplicationsoftemporalfocusingwidefieldtwophotonmicroscopy AT yewyansengelijah 6principlesandapplicationsoftemporalfocusingwidefieldtwophotonmicroscopy AT rowlandschristopher 6principlesandapplicationsoftemporalfocusingwidefieldtwophotonmicroscopy |