Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes
The lysosome degrades and recycles macromolecules, signals to the cytosol and nucleus, and is implicated in many diseases. Here, we describe a method for the rapid isolation of mammalian lysosomes and use it to quantitatively profile lysosomal metabolites under various cell states. Under nutrient-re...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Published: |
American Association for the Advancement of Science (AAAS)
2019
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| Online Access: | http://hdl.handle.net/1721.1/119871 https://orcid.org/0000-0003-4642-3706 https://orcid.org/0000-0002-1446-7256 |