A Seed Mismatch Enhances Argonaute2-Catalyzed Cleavage and Partially Rescues Severely Impaired Cleavage Found in Fish
The RNAi pathway provides both innate immunity and efficient gene-knockdown tools in many eukaryotic species, but curiously not in zebrafish. We discovered that RNAi is less effective in zebrafish at least partly because Argonaute2-catalyzed mRNA slicing is impaired. This defect is due to two mutati...
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| Format: | Article |
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Elsevier BV
2019
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| Online Access: | http://hdl.handle.net/1721.1/120144 https://orcid.org/0000-0001-9051-1696 https://orcid.org/0000-0002-4890-424X https://orcid.org/0000-0002-3872-2856 |