Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
Large synthetic genetic circuits require the simultaneous expression of many regulators. Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) direct it to bind a promoter. The programmability and specificity of RNA:DNA basepairing simplifies the generation of many or...
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Oxford University Press
2019
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| Online Access: | http://hdl.handle.net/1721.1/121036 https://orcid.org/0000-0001-8500-5836 https://orcid.org/0000-0003-0844-4776 |