Targeting individual cells by barcode in pooled sequence libraries

Transcriptional profiling of thousands of single cells in parallel by RNA-seq is now routine. However, due to reliance on pooled library preparation, targeting analysis to particular cells of interest is difficult. Here, we present a multiplexed PCR method for targeted sequencing of select cells fro...

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Bibliographic Details
Main Authors: Villani, Alexandra-Chloé, Blainey, Paul C, Ranu, Navpreet Singh, Hacohen, Nir
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Published: Oxford University Press 2019
Online Access:http://hdl.handle.net/1721.1/121041
https://orcid.org/0000-0001-5412-8200
https://orcid.org/0000-0001-7014-3830