Targeting individual cells by barcode in pooled sequence libraries
Transcriptional profiling of thousands of single cells in parallel by RNA-seq is now routine. However, due to reliance on pooled library preparation, targeting analysis to particular cells of interest is difficult. Here, we present a multiplexed PCR method for targeted sequencing of select cells fro...
Main Authors: | , , , |
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Format: | Article |
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Oxford University Press
2019
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Online Access: | http://hdl.handle.net/1721.1/121041 https://orcid.org/0000-0001-5412-8200 https://orcid.org/0000-0001-7014-3830 |