Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
CRISPR-Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both...
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
Springer Nature
2019
|
Subjects: | |
Online Access: | https://hdl.handle.net/1721.1/121998 |