Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

CRISPR-Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both...

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Bibliographic Details
Main Authors: Yin, Hao, Suresh, Sneha, Wu, Qiongqiong, Walsh, Stephen C, Ding, Junmei, Bogorad, Roman, Langer, Robert S, Anderson, Daniel Griffith
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:English
Published: Springer Nature 2019
Subjects:
Cell Biology
Molecular Biology
Online Access:https://hdl.handle.net/1721.1/121998

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