Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes
There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we...
| Principais autores: | , , , , , , , , |
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| Outros Autores: | |
| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Royal Society of Chemistry (RSC)
2020
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| Assuntos: | |
| Acesso em linha: | https://hdl.handle.net/1721.1/123502 |
| Resumo: | There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9
to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and
completely based on biologically-derived materials could offer such potential. Here we report a simple
and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently
combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines |
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