Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes
There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Royal Society of Chemistry (RSC)
2020
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| Online Access: | https://hdl.handle.net/1721.1/123502 |
| _version_ | 1811088629513060352 |
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| author | Jain, Piyush Kumar Lo, Justin H. Rananaware, Santosh Downing, Marco Panda, Apekshya Tai, Michelle Raghavan, Srivatsan Fleming, Heather Bhatia, Sangeeta N |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Jain, Piyush Kumar Lo, Justin H. Rananaware, Santosh Downing, Marco Panda, Apekshya Tai, Michelle Raghavan, Srivatsan Fleming, Heather Bhatia, Sangeeta N |
| author_sort | Jain, Piyush Kumar |
| collection | MIT |
| description | There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9
to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and
completely based on biologically-derived materials could offer such potential. Here we report a simple
and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently
combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines |
| first_indexed | 2024-09-23T14:05:01Z |
| format | Article |
| id | mit-1721.1/123502 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:05:01Z |
| publishDate | 2020 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1235022022-09-28T18:17:50Z Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes Jain, Piyush Kumar Lo, Justin H. Rananaware, Santosh Downing, Marco Panda, Apekshya Tai, Michelle Raghavan, Srivatsan Fleming, Heather Bhatia, Sangeeta N Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Broad Institute of MIT and Harvard General Materials Science There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines 2020-01-21T20:28:58Z 2020-01-21T20:28:58Z 2019-11 2019-11-08T14:53:58Z Article http://purl.org/eprint/type/JournalArticle 2040-3364 2040-3372 https://hdl.handle.net/1721.1/123502 Jain, Piyush K. et al. "Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes." Nanoscale, 11, 44 (November 2019): 21317-21323 © 2019 The Royal Society of Chemistry en http://dx.doi.org/10.1039/c9nr01786k Nanoscale Creative Commons Attribution Noncommercial 3.0 unported license https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry (RSC) Royal Society of Chemistry (RSC) |
| spellingShingle | General Materials Science Jain, Piyush Kumar Lo, Justin H. Rananaware, Santosh Downing, Marco Panda, Apekshya Tai, Michelle Raghavan, Srivatsan Fleming, Heather Bhatia, Sangeeta N Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes |
| title | Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes |
| title_full | Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes |
| title_fullStr | Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes |
| title_full_unstemmed | Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes |
| title_short | Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes |
| title_sort | non viral delivery of crispr cas9 complex using crispr gps nanocomplexes |
| topic | General Materials Science |
| url | https://hdl.handle.net/1721.1/123502 |
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