High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast

High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast

Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an e...

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Bibliographic Details
Main Author: Collins, James J.
Other Authors: Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2020
Subjects:
Biotechnology
Molecular Medicine
Applied Microbiology and Biotechnology
Bioengineering
Biomedical Engineering
Online Access:https://hdl.handle.net/1721.1/124833

Internet

https://hdl.handle.net/1721.1/124833

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