Predictable and precise template-free CRISPR editing of pathogenic variants

Predictable and precise template-free CRISPR editing of pathogenic variants

Following Cas9 cleavage, DNA repair without a donor template is generally considered stochastic, heterogeneous and impractical beyond gene disruption. Here, we show that template-free Cas9 editing is predictable and capable of precise repair to a predicted genotype, enabling correction of disease-as...

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Bibliographic Details
Main Authors: Shen, Max Walt, Arbab, Mandana, Hsu, Jonathan Yee-Ting, Worstell, Daniel, Culbertson, Sannie J., Krabbe, Olga, Cassa, Christopher A., Liu, David R., Gifford, David K., Sherwood, Richard I.
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program
Format: Article
Language:English
Published: Springer Nature 2020
Online Access:https://hdl.handle.net/1721.1/125090

Internet

https://hdl.handle.net/1721.1/125090

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