Isolating mitotic and meiotic germ cells from male mice by developmental synchronization, staging, and sorting

Isolating discrete populations of germ cells from the mouse testis is challenging, because the adult testis contains germ cells at every step of spermatogenesis, in addition to somatic cells. We present a novel method for isolating precise, high-purity populations of male germ cells. We first synchr...

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Bibliographic Details
Main Authors: Romer, Katherine A., de Rooij, Dirk G., Page, David C.
Other Authors: Whitehead Institute for Biomedical Research
Format: Article
Language:English
Published: Elsevier BV 2020
Online Access:https://hdl.handle.net/1721.1/125199