RNA-guided DNA insertion with CRISPR-associated transposases
CRISPR-Cas nucleases are powerful tools for manipulating nucleic acids; however, targeted insertion of DNA remains a challenge, as it requires host cell repair machinery. Here we characterize a CRISPR-associated transposase from cyanobacteria Scytonema hofmanni (ShCAST) that consists of Tn7-like tra...
Main Authors: | , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
American Association for the Advancement of Science (AAAS)
2020
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Online Access: | https://hdl.handle.net/1721.1/125207 |