RNA-guided DNA insertion with CRISPR-associated transposases

RNA-guided DNA insertion with CRISPR-associated transposases

CRISPR-Cas nucleases are powerful tools for manipulating nucleic acids; however, targeted insertion of DNA remains a challenge, as it requires host cell repair machinery. Here we characterize a CRISPR-associated transposase from cyanobacteria Scytonema hofmanni (ShCAST) that consists of Tn7-like tra...

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Bibliographic Details
Main Authors: Strecker, Jonathan, Ladha, Alim, Gardner, Zachary, Schmid-Burgk, Jonathan L., Makarova, Kira S., Koonin, Eugene V., Zhang, Feng
Other Authors: McGovern Institute for Brain Research at MIT
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS) 2020
Online Access:https://hdl.handle.net/1721.1/125207

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