Diversity, classification and evolution of CRISPR-Cas systems
The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2020
|
| Online Access: | https://hdl.handle.net/1721.1/125357 |
| _version_ | 1811070888211120128 |
|---|---|
| author | Koonin, Eugene V Makarova, Kira S Zhang, Feng |
| author2 | Broad Institute of MIT and Harvard |
| author_facet | Broad Institute of MIT and Harvard Koonin, Eugene V Makarova, Kira S Zhang, Feng |
| author_sort | Koonin, Eugene V |
| collection | MIT |
| description | The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with multi-subunit effector complexes in Class 1 and single-protein effector modules in Class 2. Concerted genomic and experimental efforts on comprehensive characterization of Class 2 CRISPR-Cas systems led to the identification of two new types and several subtypes. The newly characterized type VI systems are the first among the CRISPR-Cas variants to exclusively target RNA. Unexpectedly, in some of the class 2 systems, the effector protein is additionally responsible for the pre-crRNA processing. Comparative analysis of the effector complexes indicates that Class 2 systems evolved from mobile genetic elements on multiple, independent occasions. |
| first_indexed | 2024-09-23T08:43:15Z |
| format | Article |
| id | mit-1721.1/125357 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:43:15Z |
| publishDate | 2020 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1253572022-09-30T10:45:49Z Diversity, classification and evolution of CRISPR-Cas systems Koonin, Eugene V Makarova, Kira S Zhang, Feng Broad Institute of MIT and Harvard McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with multi-subunit effector complexes in Class 1 and single-protein effector modules in Class 2. Concerted genomic and experimental efforts on comprehensive characterization of Class 2 CRISPR-Cas systems led to the identification of two new types and several subtypes. The newly characterized type VI systems are the first among the CRISPR-Cas variants to exclusively target RNA. Unexpectedly, in some of the class 2 systems, the effector protein is additionally responsible for the pre-crRNA processing. Comparative analysis of the effector complexes indicates that Class 2 systems evolved from mobile genetic elements on multiple, independent occasions. 2020-05-20T18:26:37Z 2020-05-20T18:26:37Z 2017-06 2019-10-08T12:15:10Z Article http://purl.org/eprint/type/JournalArticle 1369-5274 https://hdl.handle.net/1721.1/125357 VKoonin, Eugene, Makarova, Kira S. and Zhang, Feng, "Diversity, classification and evolution of CRISPR-Cas systems." Current Opinion in Microbiology 37 (June 2017): 67-78 © 2017 en http://dx.doi.org/10.1016/j.mib.2017.05.008 Current Opinion in Microbiology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Koonin, Eugene V Makarova, Kira S Zhang, Feng Diversity, classification and evolution of CRISPR-Cas systems |
| title | Diversity, classification and evolution of CRISPR-Cas systems |
| title_full | Diversity, classification and evolution of CRISPR-Cas systems |
| title_fullStr | Diversity, classification and evolution of CRISPR-Cas systems |
| title_full_unstemmed | Diversity, classification and evolution of CRISPR-Cas systems |
| title_short | Diversity, classification and evolution of CRISPR-Cas systems |
| title_sort | diversity classification and evolution of crispr cas systems |
| url | https://hdl.handle.net/1721.1/125357 |
| work_keys_str_mv | AT koonineugenev diversityclassificationandevolutionofcrisprcassystems AT makarovakiras diversityclassificationandevolutionofcrisprcassystems AT zhangfeng diversityclassificationandevolutionofcrisprcassystems |