A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells
Microfluidic-based organ-on-a-chip assays with simultaneous coculture of multi-cell types have been widely utilized for basic research and drug development. Here we describe a novel method for a primary cell-based corneal microphysiological system which aims to recapitulate the basic functions of th...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Frontiers Media SA
2020
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| Online Access: | https://hdl.handle.net/1721.1/125467 |
| _version_ | 1826188576291815424 |
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| author | Bai, Jing Fu, Haojie Bazinet, Lauren Birsner, Amy E. D'Amato, Robert J. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Bai, Jing Fu, Haojie Bazinet, Lauren Birsner, Amy E. D'Amato, Robert J. |
| author_sort | Bai, Jing |
| collection | MIT |
| description | Microfluidic-based organ-on-a-chip assays with simultaneous coculture of multi-cell types have been widely utilized for basic research and drug development. Here we describe a novel method for a primary cell-based corneal microphysiological system which aims to recapitulate the basic functions of the in vivo cornea and to study topically applied ocular drug permeation. In this study, the protocols for isolating and cultivating primary corneal epithelial cells and endothelial cells from mouse inbred strain C57BL/6J were optimized, to allow for the development of a primary-cell based microfluidic 3D micro-engineered cornea. This tissue unit, by overcoming the limitations of 2D conventional cell culture, supports new investigations on cornea function and facilitates drug delivery testing. |
| first_indexed | 2024-09-23T08:01:49Z |
| format | Article |
| id | mit-1721.1/125467 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:01:49Z |
| publishDate | 2020 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | mit-1721.1/1254672022-09-30T01:49:30Z A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells Bai, Jing Fu, Haojie Bazinet, Lauren Birsner, Amy E. D'Amato, Robert J. Massachusetts Institute of Technology. Department of Mechanical Engineering Microfluidic-based organ-on-a-chip assays with simultaneous coculture of multi-cell types have been widely utilized for basic research and drug development. Here we describe a novel method for a primary cell-based corneal microphysiological system which aims to recapitulate the basic functions of the in vivo cornea and to study topically applied ocular drug permeation. In this study, the protocols for isolating and cultivating primary corneal epithelial cells and endothelial cells from mouse inbred strain C57BL/6J were optimized, to allow for the development of a primary-cell based microfluidic 3D micro-engineered cornea. This tissue unit, by overcoming the limitations of 2D conventional cell culture, supports new investigations on cornea function and facilitates drug delivery testing. 2020-05-26T21:27:51Z 2020-05-26T21:27:51Z 2020-04 2019-11 2020-05-18T15:09:04Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/125467 Bai, Jing et al. "A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells." Frontiers in Pharmacology 11 (April 2020): 453 en http://dx.doi.org/10.3389/fphar.2020.00453 Frontiers in Pharmacology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media SA Frontiers |
| spellingShingle | Bai, Jing Fu, Haojie Bazinet, Lauren Birsner, Amy E. D'Amato, Robert J. A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells |
| title | A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells |
| title_full | A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells |
| title_fullStr | A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells |
| title_full_unstemmed | A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells |
| title_short | A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells |
| title_sort | method for developing novel 3d cornea on a chip using primary murine corneal epithelial and endothelial cells |
| url | https://hdl.handle.net/1721.1/125467 |
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