Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic gen...

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Bibliographic Details
Main Authors: Yamano, Takashi, Zetsche, Bernd, Ishitani, Ryuichiro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Other Authors: Broad Institute of MIT and Harvard
Format: Article
Language:English
Published: Elsevier BV 2020
Online Access:https://hdl.handle.net/1721.1/125487

Internet

https://hdl.handle.net/1721.1/125487

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