Systems analysis of EGF receptor signaling dynamics with microwestern arrays
We describe microwestern arrays, which enable quantitative, sensitive and high-throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates. This method allowed us to measure 91 phosphosites on 67 proteins at six time points after stimula...
Main Authors: | , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
Springer Science and Business Media LLC
2020
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Online Access: | https://hdl.handle.net/1721.1/126134 |