Systems analysis of EGF receptor signaling dynamics with microwestern arrays

We describe microwestern arrays, which enable quantitative, sensitive and high-throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates. This method allowed us to measure 91 phosphosites on 67 proteins at six time points after stimula...

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Bibliographic Details
Main Authors: Ciaccio, Mark F, Wagner, Joel P, Chuu, Chih-Pin, Lauffenburger, Douglas A, Jones, Richard B
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2020
Online Access:https://hdl.handle.net/1721.1/126134