CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses
A key goal for cell biological analyses is to assess the phenotypes that result from eliminating a target gene. Since the early 1990s, the predominant strategy utilized in human tissue culture cells has been RNA interference (RNAi)-mediated protein depletion. However, RNAi suffers well-documented of...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Society for Cell Biology (ASCB)
2020
|
| Online Access: | https://hdl.handle.net/1721.1/126142 |