CRISPR Guide RNA Cloning for Mammalian Systems

CRISPR Guide RNA Cloning for Mammalian Systems

The outlined protocol describes streamlined methods for the efficient and cost-effective generation of Cas9-associated guide RNAs. Two alternative strategies for guide RNA (gRNA) cloning are outlined based on the usage of the Type IIS restriction enzyme BsmBI in combination with a set of compatible...

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Bibliographic Details
Main Authors: Nageshwaran, Sathiji, Chavez, Alejandro, Cher Yeo, Nan, Guo, Xiaoge, Lance-Byrne, Alissa, Tung, Angela, Collins, James J., Church, George M.
Other Authors: Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Format: Article
Language:English
Published: MyJove Corporation 2020
Online Access:https://hdl.handle.net/1721.1/126272

Internet

https://hdl.handle.net/1721.1/126272

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