A pathway for mitotic chromosome formation
Mitotic chromosomes fold as compact arrays of chromatin loops.To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a con...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2020
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| Online Access: | https://hdl.handle.net/1721.1/126370 |
| _version_ | 1826194711801495552 |
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| author | Gibcus, Johan H. Samejima, Kumiko Goloborodko, Anton Samejima, Itaru Naumova, Natalia Nubler, Johannes Kanemaki, Masato T. Xie, Linfeng Paulson, James R. Earnshaw, William C. Mirny, Leonid A Dekker, Job |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Gibcus, Johan H. Samejima, Kumiko Goloborodko, Anton Samejima, Itaru Naumova, Natalia Nubler, Johannes Kanemaki, Masato T. Xie, Linfeng Paulson, James R. Earnshaw, William C. Mirny, Leonid A Dekker, Job |
| author_sort | Gibcus, Johan H. |
| collection | MIT |
| description | Mitotic chromosomes fold as compact arrays of chromatin loops.To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed.During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops.The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold.The size of helical turns progressively increases to ~12megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding. |
| first_indexed | 2024-09-23T10:00:58Z |
| format | Article |
| id | mit-1721.1/126370 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:00:58Z |
| publishDate | 2020 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1263702022-09-26T15:10:38Z A pathway for mitotic chromosome formation Gibcus, Johan H. Samejima, Kumiko Goloborodko, Anton Samejima, Itaru Naumova, Natalia Nubler, Johannes Kanemaki, Masato T. Xie, Linfeng Paulson, James R. Earnshaw, William C. Mirny, Leonid A Dekker, Job Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Physics Mitotic chromosomes fold as compact arrays of chromatin loops.To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed.During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops.The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold.The size of helical turns progressively increases to ~12megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding. National Human Genome Research Institute (Grant HG003143) National Institutes of Health Common Fund (Grant DK107980) 2020-07-24T15:27:33Z 2020-07-24T15:27:33Z 2018-01 2017-08 2019-10-09T16:30:06Z Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 https://hdl.handle.net/1721.1/126370 Gibcus, Johan H. et al. "A pathway for mitotic chromosome formation." Science 359, 6376 (January 2018): eaao6135 © 2017 The Authors en http://dx.doi.org/10.1126/science.aao6135 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Gibcus, Johan H. Samejima, Kumiko Goloborodko, Anton Samejima, Itaru Naumova, Natalia Nubler, Johannes Kanemaki, Masato T. Xie, Linfeng Paulson, James R. Earnshaw, William C. Mirny, Leonid A Dekker, Job A pathway for mitotic chromosome formation |
| title | A pathway for mitotic chromosome formation |
| title_full | A pathway for mitotic chromosome formation |
| title_fullStr | A pathway for mitotic chromosome formation |
| title_full_unstemmed | A pathway for mitotic chromosome formation |
| title_short | A pathway for mitotic chromosome formation |
| title_sort | pathway for mitotic chromosome formation |
| url | https://hdl.handle.net/1721.1/126370 |
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