Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
Herein, we describe a protocol for the isolation of human embryonic stem cells (hESCs)-derived vascular cells at various stages of development. The cells are isolated from 10 to 15-d-old human embryoid bodies (EBs) cultured in suspension. After dissociation, cells are labeled with anti-CD34 or anti-...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Nature America, Inc
2020
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| Online Access: | https://hdl.handle.net/1721.1/126579 |