Mechanisms for curing yeast prions
Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Published: |
Multidisciplinary Digital Publishing Institute
2020
|
| Online Access: | https://hdl.handle.net/1721.1/127693 |
| _version_ | 1826203114738286592 |
|---|---|
| author | Greene, Lois E. Saba, Farrin Silberman, Rebecca Estelle Zhao, Xiaohong |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Greene, Lois E. Saba, Farrin Silberman, Rebecca Estelle Zhao, Xiaohong |
| author_sort | Greene, Lois E. |
| collection | MIT |
| description | Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the misfolded prion proteins are assembled into amyloid fibers or seeds, which are constantly severed and transmitted to daughter cells. To cure prions in yeast, it is necessary to eliminate all the prion seeds. Multiple mechanisms of curing have been found including inhibiting severing of the prion seeds, gradual dissolution of the prion seeds, asymmetric segregation of the prion seeds between mother and daughter cells during cell division, and degradation of the prion seeds. These mechanisms, achieved by using different protein quality control machinery, are not mutually exclusive; depending on conditions, multiple mechanisms may work simultaneously to achieve curing. This review discusses the various methods that have been used to differentiate between these mechanisms of curing. |
| first_indexed | 2024-09-23T12:31:53Z |
| format | Article |
| id | mit-1721.1/127693 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T12:31:53Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1276932022-10-01T09:36:33Z Mechanisms for curing yeast prions Greene, Lois E. Saba, Farrin Silberman, Rebecca Estelle Zhao, Xiaohong Koch Institute for Integrative Cancer Research at MIT Prions are infectious proteins that self-propagate by changing from their normal folded conformation to a misfolded conformation. The misfolded conformation, which is typically rich in β-sheet, serves as a template to convert the prion protein into its misfolded conformation. In yeast, the misfolded prion proteins are assembled into amyloid fibers or seeds, which are constantly severed and transmitted to daughter cells. To cure prions in yeast, it is necessary to eliminate all the prion seeds. Multiple mechanisms of curing have been found including inhibiting severing of the prion seeds, gradual dissolution of the prion seeds, asymmetric segregation of the prion seeds between mother and daughter cells during cell division, and degradation of the prion seeds. These mechanisms, achieved by using different protein quality control machinery, are not mutually exclusive; depending on conditions, multiple mechanisms may work simultaneously to achieve curing. This review discusses the various methods that have been used to differentiate between these mechanisms of curing. Intramural Program of the National Heart Lung Blood Institute of the National Institutes of Health (1ZIAHL000516) 2020-09-25T19:18:37Z 2020-09-25T19:18:37Z 2020-09 2020-09-07T22:02:09Z Article http://purl.org/eprint/type/JournalArticle 1422-0067 https://hdl.handle.net/1721.1/127693 Greene, Lois E. et al. "Mechanisms for curing yeast prions." International Journal of Molecular Sciences 21, 18 (September 2020): 6536 ©2020 Author(s) http://dx.doi.org/10.3390/ijms21186536 International Journal of Molecular Sciences Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Greene, Lois E. Saba, Farrin Silberman, Rebecca Estelle Zhao, Xiaohong Mechanisms for curing yeast prions |
| title | Mechanisms for curing yeast prions |
| title_full | Mechanisms for curing yeast prions |
| title_fullStr | Mechanisms for curing yeast prions |
| title_full_unstemmed | Mechanisms for curing yeast prions |
| title_short | Mechanisms for curing yeast prions |
| title_sort | mechanisms for curing yeast prions |
| url | https://hdl.handle.net/1721.1/127693 |
| work_keys_str_mv | AT greeneloise mechanismsforcuringyeastprions AT sabafarrin mechanismsforcuringyeastprions AT silbermanrebeccaestelle mechanismsforcuringyeastprions AT zhaoxiaohong mechanismsforcuringyeastprions |