Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
Prolonged Cas9 activity can hinder genome engineering as it causes off-target effects, genotoxicity, heterogeneous genome-editing outcomes, immunogenicity, and mosaicism in embryonic editing - issues which could be addressed by controlling the longevity of Cas9. Though some temporal controls of Cas9...
Main Authors: | , , , , , , , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
American Chemical Society (ACS)
2020
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Online Access: | https://hdl.handle.net/1721.1/128916 |