Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes

Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes

Prolonged Cas9 activity can hinder genome engineering as it causes off-target effects, genotoxicity, heterogeneous genome-editing outcomes, immunogenicity, and mosaicism in embryonic editing - issues which could be addressed by controlling the longevity of Cas9. Though some temporal controls of Cas9...

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Bibliographic Details
Main Authors: Sreekanth, Vedagopuram, Zhou, Qingxuan, Kokkonda, Praveen, Bermudez-Cabrera, Heysol C., Lim, Donghyun, Law, Benjamin K., Holmes, Benjamin Ray, Chaudhary, Santosh K., Pergu, Rajaiah, Leger, Brittany S., Walker, James A., Gifford, David K, Sherwood, Richard I., Choudhary, Amit
Other Authors: McGovern Institute for Brain Research at MIT
Format: Article
Language:English
Published: American Chemical Society (ACS) 2020
Online Access:https://hdl.handle.net/1721.1/128916

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